Thermo Fisher Scientific HLA-DR Monoclonal Antibody (YE2/36 HLK), Biotin

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1.0 µg/1x10^6 cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

YE2/36 HLK

Immunogen

EHR-B Ramos cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HLA-DR,uniProtId:P01903-1,ncbiNodeId:9606,antigenRange:1-254,antigenLength:254,antigenImageFileName:MA5-18017_HLA-DR_P01903-1_Rat.svg,antigenImageFileNamePDP:MA5-18017_HLA-DR_P01903-1_Rat_PDP.jpeg,sortOrder:1},{targetFamily:HLA-DR,uniProtId:P79483-1,ncbiNodeId:9606,antigenRange:1-266,antigenLength:266,antigenImageFileName:MA5-18017_HLA-DR_P79483-1_Rat.svg,antigenImageFileNamePDP:MA5-18017_HLA-DR_P79483-1_Rat_PDP.jpeg,sortOrder:2},{targetFamily:HLA-DR,uniProtId:P01911-1,ncbiNodeId:9606,antigenRange:1-266,antigenLength:266,antigenImageFileName:MA5-18017_HLA-DR_P01911-1_Rat.svg,antigenImageFileNamePDP:MA5-18017_HLA-DR_P01911-1_Rat_PDP.jpeg,sortOrder:3},{targetFamily:HLA-DR,uniProtId:P04233-1,ncbiNodeId:9606,antigenRange:1-296,antigenLength:296,antigenImageFileName:MA5-18017_HLA-DR_P04233-1_Rat.svg,antigenImageFileNamePDP:MA5-18017_HLA-DR_P04233-1_Rat_PDP.jpeg,sortOrder:4},{targetFamily:HLA-DR,uniProtId:P13762-1,ncbiNodeId:9606,antigenRange:1-266,antigenLength:266,antigenImageFileName:MA5-18017_HLA-DR_P13762-1_Rat.svg,antigenImageFileNamePDP:MA5-18017_HLA-DR_P13762-1_Rat_PDP.jpeg,sortOrder:5},{targetFamily:HLA-DR,uniProtId:Q30154-1,ncbiNodeId:9606,antigenRange:1-266,antigenLength:266,antigenImageFileName:MA5-18017_HLA-DR_Q30154-1_Rat.svg,antigenImageFileNamePDP:MA5-18017_HLA-DR_Q30154-1_Rat_PDP.jpeg,sortOrder:6}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

View additional formats

• FITC
• PE
• Request custom conjugation

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 4-5mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539401

Product Specific Information

MA5-18017 targets HLA-DR in FACS applications and shows reactivity with Human samples.

The MA5-18017 immunogen is eHR-B Ramos cells.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.