Invitrogen™

Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The자세히 알아보기

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.

Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells
• Treg cells can be expanded with Dynabeads™ CD3/CD28 Treg Expander and remain
Foxp3+.
• Treg cells can be used in any downstream applications (e.g., suppression assays)

Methodology
The isolation procedure includes the following steps:
• Negative isolation of untouched human CD4+ T-cells from PBMC.
• Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset.
• Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD™

Starting sample
PBMC

사양

세포 유형T Cells (Regulatory)

제품 유형Cell Isolation Kit

분리 기술Depletion, then positive isolation

셀 수Processes ∼1 x 109 cells total

출력 생존력>95%

제품라인DETACHaBEAD™, DYNAL™, Dynabeads™, FlowComp™

순도 또는 품질 등급Research Grade

수량2 x 5 mL

샘플 종류PBMC

배송 조건Room Temperature

시작 물질 셀 수1 x 108 PBMCs per isolation

타겟 종Human

Unit SizeEach