Takara Yeast minimal media—SD base

These high-quality yeast growth media can be used together with dropout (DO) supplements for confirming auxotrophic phenotypes and selecting yeast transformants. They are ideal for use with Matchmaker Gold systems and other yeast one-hybrid systems or yeast two-hybrid systems.

Minimal SD bases

Minimal synthetic defined (SD) bases include a yeast nitrogen base, ammonium sulfate, and a glucose as a carbon source. Amino acids are not added so dropout (DO) supplements must be added separately to the Minimal SD Base to make a synthetic, defined medium lacking the specified nutrients allowing for selection of transformed yeast clones. The SD bases are available with or without agar premixed in the medium. Each package of Minimal SD Base makes 10 liters of medium.