Thermo Fisher Scientific LC3A/LC3B Polyclonal Antibody, DyLight 550

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Flow Cytometry (Flow)

Assay-Dependent

-

ELISA (ELISA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Amphibian, Dog, Fish, Human, Mouse, Plant, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LC3A/LC3B,uniProtId:Q9GZQ8-1,ncbiNodeId:9606,antigenRange:25-121,antigenLength:125,antigenImageFileName:PA5-22733_LC3ALC3B_Q9GZQ8-1_Rabbit.svg,antigenImageFileNamePDP:PA5-22733_LC3ALC3B_Q9GZQ8-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:LC3A/LC3B,uniProtId:A6NCE7-1,ncbiNodeId:9606,antigenRange:25-121,antigenLength:125,antigenImageFileName:PA5-22733_LC3ALC3B_A6NCE7-1_Rabbit.svg,antigenImageFileNamePDP:PA5-22733_LC3ALC3B_A6NCE7-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

DyLight™ 550 DyLight™ 550 DyLight™ 550

Excitation/Emission Max

556/570 nm View spectra

Form

Liquid

Concentration

0.79 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

50mM sodium borate

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154000

Product Specific Information

The target sequence has 100% sequence homology with xenopus, bovine and zebrafish.

Target Information

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.