
Thermo Fisher Scientific Mouse anti-Human IgM Secondary Antibody
인간 IgM 검출을 위한 마우스 단클론 2차 항체로, WB·Flow·ELISA 등 다양한 면역실험에 사용 가능. 비결합형 액상 형태이며, 높은 특이성과 신뢰성 제공. 단백질 A로 정제되어 안정적이며 연구용으로 적합.
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Thermo Fisher Scientific Mouse anti-Human IgM Secondary Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Flow Cytometry (Flow) | 1 µg/mL |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | Assay-dependent |
| Radioimmune Assays (RIA) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | P5E2 |
| Immunogen | Purified Human Serum IgM |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 2–8°C (up to 6 months), -20°C (long-term) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153935 |
| Target | IgM |
| Antibody Form | Whole Antibody |
Product Specific Information
- By ELISA and WB, MII0401 does not react with Human IgM or Human IgA.
- MII0401 is also sold as a smaller package size (Product # MA5-14729).
- MII0401 was formerly sold as a Seradyn product.
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer versatility for multiple detection systems (e.g., HRP, AP, fluorescence) and provide greater sensitivity via signal amplification as multiple secondary antibodies can bind to a single primary antibody.
Typically, they are generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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