
Thermo Fisher Scientific Peroxidase Polyclonal Antibody
Western blot, ELISA, IP 등 다양한 응용에 적합한 다클론 항-페록시다제 항체. 양(IgG) 유래로 고순도 정제. HRP 검출용 연구 시약으로 높은 안정성과 재현성 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA (ELISA) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peroxidase (Horseradish) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-PEROXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis against the specified buffer.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Peroxidase (Horseradish). Cross-reactivity against peroxidase from other tissues and species may occur but has not been specifically determined.
Target Information
HRP (Horseradish Peroxidase) is an enzyme derived from the root of the horseradish plant. It catalyzes the oxidation of various substrates in the presence of hydrogen peroxide.
HRP is widely used as a detection enzyme in immunoassays such as ELISA and Western blot due to its high sensitivity and stability. It can also serve as a tracer in enzyme-linked immunosorbent assays and as a labeling agent in fluorescent microscopy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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