Thermo Fisher Scientific RTCA Polyclonal Antibody
RTCA 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC(P), ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol buffer에 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human RTCA. Recombinant protein control fragment (Product #RP-94251) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2646856 |
Product Specific Information
Immunogen sequence:
LFAASPSELH LKGGTNAEMA PQIDYTVMVF KPIVEKFGFI FNCDIKTRGY YPKGGGEVIV RMSPVKQLNP INLTERGCVT KIY
Highest antigen sequence identity to the following orthologs:
- Mouse: 89%
- Rat: 90%
Target Information
RTCD1 catalyzes the conversion of 3′-phosphate to a 2′,3′-cyclic phosphodiester at the end of RNA.
The mechanism of action occurs in three steps:
- Adenylation of the enzyme by ATP
- The enzyme acts on RNA-N3′P to produce RNA-N3′PP5′A
- A non-catalytic nucleophilic attack by the adjacent 2′ hydroxyl on the phosphorus in the diester linkage produces the cyclic end product
The biological role of this enzyme is unknown, but it is likely to function in aspects of cellular RNA processing. Two isoforms are known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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