Thermo Fisher Scientific RTCA Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human RTCA. Recombinant protein control fragment (Product #RP-94251). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RTCA,uniProtId:O00442-1,ncbiNodeId:9606,antigenRange:111-193,antigenLength:366,antigenImageFileName:PA5-55742_RTCA_O00442-1_Rabbit.svg,antigenImageFileNamePDP:PA5-55742_RTCA_O00442-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2646856

Product Specific Information

Immunogen sequence: LFAASPSELH LKGGTNAEMA PQIDYTVMVF KPIVEKFGFI FNCDIKTRGY YPKGGGEVIV RMSPVKQLNP INLTERGCVT KIY

Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 90%.

Target Information

RTCD1 catalyzes the conversion of 3-phosphate to a 2, 3-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3P to produce RNA-N3PP5A; (C) a non catalytic nucleophilic attack by the adjacent 2`hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. There are two named isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.