Himedia GranuloSep GSM 1119

It is based on the adapted method of isolating granulocyte using centrifugation techniques by Bøyum in which defibrinated blood is layered on a solution of sodium diatrizoate and polysucrose and centrifuged at low speeds for 30 minutes. A double gradient is formed by layering an equal volume of HiSepTM 1077 over GranuloSepTM GSM 1119. Whole blood is carefully layered on to the upper HiSepTM 1077 medium. Differential migration following centrifugation results in the formation of several cell layers. Cells of the granulocytic series are found at the 1077/1119 interphase whereas lymphocyte, other mononuclear cells and platelets are found at plasma/1077 interphase. GranuloSepTM GSM 1119 is a solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.119 ± 0.0010g/ml. When combined with HiSep™ LSM 1077, it permits separation of mononuclear cells and granulocytes from defibrinated EDTA or heparin treated human blood.