Thermo Fisher Scientific NKX3.1 Monoclonal Antibody (NKX3.1, 2576)
인간 NKX3.1 단백질을 인식하는 마우스 단클론 항체로, 면역조직화학(IHC) 및 펩타이드 어레이에 사용 가능. 단백질 A/G로 정제된 액상 항체이며, 보존제 미첨가. 전립선 특이적 종양 억제 단백질 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2c, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NKX3.1, 2576 |
| Immunogen | Recombinant fragment (around aa 92–224) of human NKX3.1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage conditions | -20°C or -80°C if preferred |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is tested in direct ELISA.
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
NKX3-1 is a homeodomain-containing transcription factor and a putative prostate tumor suppressor.
It is expressed in a largely prostate-specific and androgen-regulated manner.
Loss of NKX3-1 protein expression is commonly observed in human prostate carcinomas and prostatic intraepithelial neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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