Thermo Fisher Scientific CRACR2A (EFCAB4B) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:400

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)KSEKEQFLLKDTER, corresponding to amino acid residues 245-258 of human CRACR2A, Intracellular if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EFCAB4B,uniProtId:Q9BSW2-2,ncbiNodeId:9606,antigenRange:245-258,antigenLength:731,antigenImageFileName:ACC-324-200UL_EFCAB4B_Q9BSW2-2_Rabbit.svg,antigenImageFileNamePDP:ACC-324-200UL_EFCAB4B_Q9BSW2-2_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.85 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

CRACR2A is a Ca(2+)-binding protein that plays a key role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. CRACR2A acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. Further, CRACR2A regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, CRACR2A dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.