Thermo Fisher Scientific FITC Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg

View 4 publications 4 publications

Immunohistochemistry (IHC)

Assay-dependent

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 4 publications 4 publications

ELISA (ELISA)

0.5-1.0 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Immunomicroscopy (IM)

-

View 1 publication 1 publication

In Situ Hybridization (ISH)

-

View 1 publication 1 publication

Affinity Purification (AP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FITC

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2533978

Product Specific Information

This antibody was purified from serum of rabbit hyper-immunized with FITC conjugated to KLH. The antibody was purified by antigen-specific matrix. The antibody reacts with FITC but not the carrier protein. Useful as a secondary antibody in a wide range of immunoassays including: ELISA, tissue and cell staining, immunoprecipitation, and western blotting. The protein concentration is determined based on an E (1%) = 14 at 280 nm.

Target Information

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.