Thermo Scientific™

Bovine Genotypes Panel 2.2 Kit

Thermo Scientific Bovine Genotypes Panel 2.2 Kit is offered for cattle parentage testing and identification using Short Tandem Repeat (STR)자세히 알아보기

Thermo Scientific Bovine Genotypes Panel 2.2 Kit is offered for cattle parentage testing and identification using Short Tandem Repeat (STR) DNA loci.

The Bovine Genotypes Panel 2.2 allows co-amplification of the 6 microsatellites in a single multiplex PCR reaction. One primer from each primer pair is end-labeled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated electrophoresis instrument (see Figure 2). In addition, the kit includes bovine control DNA for verification of acceptable PCR and electrophoresis conditions. Reagents and protocols of the Bovine Genotypes Panel 2.2 have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of plus-A peaks or split peaks typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the plus-A peaks.

Thermo Scientific Bovine Genotypes Panel 2.2 encompasses the following six STR loci: SPS113, RM067, CSRM60, MGTG4B, CSSM66, and ILSTS006. These are among the list of loci recommended by the International Society for Animal Genetics (ISAG) and the Food and Agriculture Organization of the United Nations (FAO) for genetic studies of domestic animals.

The Bovine Genotypes Panel 2.2 is intended to be used as a complement to the Bovine Genotypes Panel 1.2 when more STR loci are required for resolving a parentage case. The microsatellite alleles of the Bovine Genotypes Panel 2.2 kit can be separated and detected in the same single electrophoresis injection with the microsatellite alleles of the Bovine Genotypes Panel 1.2 kit (see Figure 2).

Highlights

• Various kit choices that include STR loci recommended by ISAG
• Single multiplex reaction - less pipetting steps
• Optimized reagents
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calling represents true alleles (no plus-A peaks)

This product has been developed in collaboration with Maxxam Analytics Inc.

사양

검출 방법Capillary Electrophoresis

용도(애플리케이션)PCR

반응 수500 Reactions

제품 유형Bovine Genotypes Panel 2.2 Kit

수량500 reactions

타겟Bovine Genotyping and Parentage Testing

기술STR (Short Tandem Repeat) Analysis

Unit SizeEach