Invitrogen™

_Funga_Light™ Yeast CFDA, AM/Propidium Iodide Vitality Kit

The FungaLight™ Yeast CFDA, AM / Propidium Iodide Vitality Kit combines a cell permeable esterase substrate with a membrane integrity자세히 알아보기

The _FungaLight_™ Yeast CFDA, AM / Propidium Iodide Vitality Kit combines a cell permeable esterase substrate with a membrane integrity indicator to evaluate the vitality of yeast cells by flow cytometry or microscopy. The acetoxymethyl ester (AM) of the esterase substrate 5-carboxyfluorescein diacetate (CFDA) allows this reagent to permeant cell membranes Once inside the cell, the lipophilic blocking and diacetate groups are cleaved by nonspecific esterases, resulting in a fluorescent, charged form that leaks out of cells very slowly. In contrast, the membrane integrity indicator, propidium iodide penetrates yeast with damaged membranes. With an appropriate mixture of the CFDA, AM and propidium iodide stains, esterase-active yeast with intact cell membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.

사양

세포 유형Yeast Cells

검출 방법Fluorescence

용도(애플리케이션)Viability Assay

용도 (장비)Fluorescence Microscope, Flow Cytometer

제품 유형AM/Propidium Iodide Vitality Kit

염료 유형Other Label(s) or Dye(s)

방출PI: 490⁄635, CFDA: 492⁄517

형태Solid

형식Tube(s), Slide(s)

제품라인FungaLight™

수량200 kit

배송 조건Room Temperature

용해도DMSO (Dimethylsulfoxide)

Unit SizeEach