Thermo Fisher Scientific Rabbit anti-Human IgA1 Recombinant Secondary Antibody
Human IgA1 특이적 Rabbit Recombinant Secondary Antibody로 높은 특이성과 민감도를 제공. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합. Lot 간 일관성 확보 및 동물 유래 성분 없는 포뮬레이션. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,500 |
| Immunohistochemistry (IHC) | 0.1–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 50–200 ng/well (Capture), 0.05–0.2 µg/mL (Detect) |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | RM124 |
| Immunogen | Peptide corresponding to Human IgA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2665315 |
| Target | IgA1 |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody reacts to human IgA1 and shows very slight cross-reactivity with IgA2.
No cross-reactivity with human IgG, IgM, IgD, or IgE.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal production candidates.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Anti-Human secondary antibodies are affinity-purified reagents with well-characterized specificity for human immunoglobulins.
They are useful for detection, sorting, or purification of target molecules.
Secondary antibodies enable versatile detection systems (HRP, AP, fluorescence) and provide enhanced sensitivity via signal amplification.
Typically generated by immunizing host animals with pooled immunoglobulins from the target species, they can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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