Thermo Scientific™

Luminaris HiGreen qPCR Master Mix, high ROX

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Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
—Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
—Sensitivity: ingle copy detection
—Wide linear range: accurate quantification across 9 orders of magnitude
—Reproducibility and convenience: ready-to-use 2X master mix

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris HiGreen High ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.

사양

검출 방법SYBR

GC-Rich PCR PerformanceHigh

PCR 방법qPCR

중합효소Taq DNA Polymerase

반응 속도Standard

용도(장비)7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System

제품라인Luminaris

제품 유형qPCR Master Mix

충분250 Reactions

용도(애플리케이션)Gene Expression

농도2X

Fidelity (Taq 대비)2 X

반응 수1250 Reactions

샘플 종류DNA, dsDNA

Unit Size1250 reactions