Invitrogen™

SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of자세히 알아보기

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR. This one-step formulation allows for both cDNA synthesis and PCR amplification to be performed in a single tube using gene-specific primers and target RNAs from either total RNA or mRNA. It also allows for the detection of a wide range of RNA targets (up to 10 kb in length) at variable concentrations (1 pg to 1 μg of total RNA). This system consists of two major components: SuperScript III RT/Platinum Taq High Fidelity enzyme mix and 2X reaction mix.

SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA and can be used to synthesize cDNA from total RNA. Platinum Taq DNA Polymerase High Fidelity is a an enzyme mixture composed of recombinant Taq DNA polymerase, Pyrococcus species GB–D polymerase, and Platinum Taq antibodies, which block polymerase activity at ambient temperatures enabling hot start PCR.

The 2X reaction mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers.

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity offers:
• High-fidelity end-point detection and analysis of RNA molecules by RT-PCR
• PCR amplification and cDNA synthesis in a single tube
• Detection of a wide range of RNA targets from 300 bp to 10 kb
• Increased specificity with wide cDNA synthesis temperature range of 45−60°C

Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System or SuperScript IV One-Step RT-PCR System with ezDNase. The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.

사양

검출 방법Gel Electrophoresis

형식One-Step RT-PCR System

GC-Rich PCR PerformanceHigh

PCR 방법1-step RT-PCR

중합효소Platinum Taq High Fidelity

반응 속도30 min.

기술1-Step RT-PCR

최적 반응 온도50°C

수량25 rxns

역전사 효소SuperScript III

리보뉴클레아제 H 활성Reduced

배송 조건Dry Ice

최종 제품 유형PCR Amplified cDNA

핫 스타트Built-In Hot Start

반응 수25 Reactions

반응 형식Premixed Components

시약 유형Reverse Transcription

크기(최종 제품)Up to 10 kb

시작 물질RNA

Unit SizeEach