Thermo Fisher Scientific HSF2 Monoclonal Antibody (3E2)
HSF2 단백질을 인식하는 Thermo Fisher Scientific의 Rat monoclonal antibody(3E2)입니다. Western blot, ICC/IF, IP 등 다양한 응용에 사용 가능하며, 인간, 마우스, 비인간 영장류에 반응합니다. 단백질 A로 정제된 액상 항체로, 4°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200 | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Immunoprecipitation (IP) | 1:400 | - |
| Gel Shift (GS) | - | View 1 publication |
Product Specifications
| Category | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3E2 |
| Immunogen | Recombinant mouse HSF2 protein (aa 1–517) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2279906 |
Product Specific Information
Recommended positive controls: LS174T, SKBR-3, or NIH-3T3 cells.
Target Information
Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated as heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcriptional level.
Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein levels.
HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA-binding activity, and is translocated to the nucleus.
HSF2 activity is associated with differentiation and development and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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