Thermo Fisher Scientific HSF2 Monoclonal Antibody (3E2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Immunoprecipitation (IP)

1:400

-

Gel Shift (GS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

3E2

Immunogen

Recombinant mouse HSF2 protein (aa1-517) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSF2,uniProtId:P38533-1,ncbiNodeId:10090,antigenRange:1-517,antigenLength:535,antigenImageFileName:MA1-91597_HSF2_P38533-1_Rat.svg,antigenImageFileNamePDP:MA1-91597_HSF2_P38533-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.1% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2279906

Product Specific Information

Recommended positive controls: LS174T, SKBR-3, or NIH-3T3 Cells.

Target Information

Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.