Thermo Fisher Scientific Phospho-ERK1/2 (Thr202,Tyr204, Thr185,Tyr187) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human Extracellular Signal-Regulated Kinase-1 surrounding threonine 202 and tyrosine 204 (MAPK3) and Human Extracellular Signal-Regulated Kinase-2 surrounding threonine 185 and tyrosine 187 (MAPK1) when the threonine and tyrosine residues are phosphorylated. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ERK1/2,uniProtId:P27361-1,ncbiNodeId:9606,antigenRange:202|204,antigenLength:379,antigenImageFileName:A303-608A_ERK12_P27361-1_Rabbit.svg,antigenImageFileNamePDP:A303-608A_ERK12_P27361-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:ERK1/2,uniProtId:P28482-1,ncbiNodeId:9606,antigenRange:185|187,antigenLength:360,antigenImageFileName:A303-608A_ERK12_P28482-1_Rabbit.svg,antigenImageFileNamePDP:A303-608A_ERK12_P28482-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

phosphate/tris citrate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, D. melanogaster, X. laevis and Bovine

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.