Gibco™

DMEM/F-12, powder

Dulbecco`s Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of자세히 알아보기

Dulbecco`s Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM / F-12 is modified as follows:

With	Without
• L-glutamine	• HEPES
• Phenol Red	• Sodium Bicarbonate

The complete formulation is available.

Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Hams F-12. This formulation combines DMEMs high concentrations of glucose, amino acids and vitamins with F-12`s wide variety of components.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco™ DMEM / F-12 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco™ DMEM / F-12 product made in our Scotland facility (32500-035). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

This product is not used for in vitro diagnostic purpose in some countries.

사양

세포주MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts

분류Animal Origin-free

형태Powder

제품 유형DMEM (Dulbecco`s Modified Eagle Medium)/F-12

Serum LevelStandard Serum Supplementation

유통 기한36 Months From Date of Manufacture

멸균Sterile-filtered

첨가제 포함High Glucose, Glutamine, Phenol Red, Sodium Pyruvate

첨가제 없음No HEPES

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™

수량10 x 1 L

배송 조건Room Temperature

Unit SizeEach