Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (93), PerCP-Cyanine5.5, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test
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Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 710
• NovaFluor Red 755
• NovaFluor Yellow 690
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32.
CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are low-affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported:
This 93 antibody is reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells.
Use ≤ 0.125 µg per test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test (empirically determined).
Antibody titration recommended for optimal performance.

Excitation: 488 nm
Emission: 695 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD16 is a 50–65 kDa cell surface molecule existing in two forms: a transmembranous form (NK cells, some T cells) and a phosphatidylinositol-linked form (granulocytes).
It serves as a low-affinity receptor for IgG (FcR III) and mediates ADCC by NK cells.

CD32 is a 40 kDa glycoprotein acting as a low-affinity receptor for IgG (Fc gamma RII).
It mediates endocytosis, secretion activation, cytotoxicity, and immunomodulation.
CD32 is expressed by B cells, monocytes, granulocytes, and platelets, and is involved in immune complex phagocytosis and regulation of antibody production by B cells.
Variants of CD32 may influence susceptibility to systemic lupus erythematosus (SLE).
Several transcript variants encoding different isoforms of CD32 have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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