Thermo Fisher Scientific H4K5ac Polyclonal Antibody
H4K5ac 폴리클로날 항체로 Western blot, ELISA, ChIP 분석에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, Rabbit IgG로 제작되었습니다. 합성 아세틸화 펩타이드 항원을 사용한 고순도 항체로, -20°C에서 보관하며 동결/해동 반복을 피해야 합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | |
| ELISA | 1 µg/mL | |
| ChIP assay (ChIP) | 5 µg antibody for 5–10 µg of Chromatin |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic acetylated peptide around K5 of human Histone H4 (NP_001029249.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.395 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914294 |
Product Specific Information
- Positive test controls include: HeLa, NIH/3T3, C6
- Immunogen sequence: GRGKG
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher-order structures.
This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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