Thermo Fisher Scientific EB1 Polyclonal Antibody
EB1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태. 미세소관 동역학 및 세포 극성 연구에 활용.
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Thermo Fisher Scientific EB1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human MAPRE1. Recombinant protein control fragment (Product #RP-102164). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789268 |
Product Specific Information
Immunogen sequence:
VAPSLVAPAL NKPKKPLTSS SAAPQRPIST QRTAAAPKAG PGVVRKNPGV GNGDDEAAEL MQQVNVLKLT VEDLEKERDF YFGKLRNIEL ICQENEGEND PVLQRIVDIL YATDEGFVIP DE
Target Information
EB1 family proteins are evolutionarily conserved proteins that bind microtubule plus-ends and centrosomes and regulate the dynamics and organization of microtubules. The MAPRE genes encode the EB1 family proteins. The human MAPRE genes MAPRE1, MAPRE2, and MAPRE3 code for EB1, RP1, and EBF3 proteins, respectively.
EB1 family members appear to be expressed ubiquitously. The EB1 (MAPRE1) gene, at 20q11.2, is fused to MLL in an adult patient with pro-B acute lymphoblastic leukemia. EB1 is a microtubule-associated protein that interacts with the colorectal adenomatous polyposis coli tumor-suppressor protein and plays important roles in regulating microtubule dynamics, cell polarity, and chromosome stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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