Thermo Fisher Scientific ECS (DYKDDDDK) Tag Polyclonal Antibody, Agarose

Applications

Tested Dilution

Publications

Immunoprecipitation (IP)

15 - 25 µL gel slurry/0.1 - 1 mg of protein lysate

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Product Specifications

Species Reactivity

Tag

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ECS (DYKDDDDK), Enterokinase Cleavage Site (DYKDDDDK) conjugated to KLH.

Conjugate

Agarose Agarose Agarose

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 6.8 to 7.4

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Goats were immunized with ECS (DYKDDDDK), Enterokinase Cleavage Site (DYKDDDDK) conjugated to KLH.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.