Himedia Accutase® 1X Accutase® enzymes in Dulbecco`s Phosphate Buffered Saline w/ Phenol red Sterile filtered

Accutase™ is a direct replacement for trypsin solution. It is useful for routine detachment of cells from standard tissue culture plastic ware and adhesion coated plastic ware. Accutase™ performs exceptionally well in detaching cells for the analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry. Cell lines tested for Accutase™ application include fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251, D54, HT1080 fibrosarcoma cells, Sf9 insect cells, human embryonic stem cells, human mesenchymal stem cells and human neural stem cells. Accutase™ does not contain mammalian or bacterial derived products. Accutase™ is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. 1X Accutase™ enzymes in Dulbecco`s Phosphate Buffered Saline containing 0.5mM EDTA sodium salt.