Thermo Fisher Scientific AKT1 Monoclonal Antibody (OTI1G5), TrueMAB
AKT1 단백질을 인식하는 Mouse Monoclonal 항체로, Western blot, IHC, ICC, Flow Cytometry에 사용 가능. Human, Mouse, Rat, Dog 반응성. Lyophilized 형태로 제공되며, PBS buffer에 보관. 세포 생존 및 신호전달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G5 |
| Immunogen | Full-length human recombinant AKT1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. Activated AKT1 inhibits apoptosis and promotes cell cycle progression by phosphorylating multiple targets across various cell types, including cancer cells.
It is activated by insulin, PI3K, IGF1, and other growth factors.
AKT1 promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of targets such as forkhead transcription factors and caspase-9.
The AKT pathway is a major focus in cancer drug discovery.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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