Thermo Fisher Scientific CD8 alpha Monoclonal Antibody (KT15), FITC
CD8α 단백질을 인식하는 쥐 유래 단클론 항체로, FITC 형광 표지되어 있습니다. Flow cytometry 및 IHC에 사용 가능하며, 단기 4°C, 장기 -20°C 보관이 권장됩니다. 면역세포 표지 및 T세포 분석 연구에 적합합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | Neat–1:10 | View 5 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | KT15 |
| Immunogen | T cell clone, C6 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538242 |
Additional Formats
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family involved in T cell signal transduction, differentiation, and activation.
CD8 is primarily expressed on T cells as a disulfide-linked heterodimer of CD8α and CD8β, functioning as a co-receptor with the T cell receptor (TCR) for MHC class I molecules.
CD8α homodimers are also found on subsets of T cells, NK cells, monocytes, and dendritic cells.
Upon MHC-I/peptide ligation by antigen-presenting cells, CD8 recruits Lck kinase, leading to lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation.
Activated CTLs are essential for pathogen and tumor clearance, and their differentiation is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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