Thermo Scientific™

Verso 1-step RT-qPCR Mix, ROX

The Thermo Scientific Verso 1-step RT-qPCR ROX Mix Kit for probe chemistry offers a quick and simple way to detect자세히 알아보기

The Thermo Scientific Verso 1-step RT-qPCR ROX Mix Kit for probe chemistry offers a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

Features of the Verso 1-step RT-qPCR ROX Mix Kit:

• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and qPCR steps performed in single tube—convenience and reduced contamination risk
• Use of the entire RT reaction as template for qPCR—improved sensitivity
• Verso RT enzyme is highly sensitive with broad dynamic range—accurate detection of RNA from 1 pg-1 ug
• Verso RT enzyme has a wide working temperature range (42 to 57°C)—improved success with GC-rich and other difficult templates
• Contains an inert blue dye for ease of pipetting during reaction set up

An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal when no cDNA storage is necessary for subsequent experiments. The approach minimizes the risk of contamination and improves reproducibility by reducing sample handling.

Applications

• Gene expression analysis
• RNAi validation
• Microarray validation
• Pathogen detection
• Genetic testing
• Disease research

사양

검출 방법Primer-probe

형식Tube

GC-Rich PCR PerformanceHigh

PCR 방법1-step RT-qPCR

용도(장비)7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System

제품라인Verso

제품 유형1-Step QRT-PCR Kit w/ROX

수량200 x 25 μL Reactions

충분200 Reactions

용도(애플리케이션)Gene Expression, Pathogen Detection

반응 수200 Reactions

샘플 종류RNA

Unit SizeEach