Gibco™

Advanced RPMI 1640 Medium

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells자세히 알아보기

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.

This Advanced RPMI 1640 is manufactured as follows:

With	Without
• Glucose	• L-glutamine
• Non Essential Amino Acids	• HEPES
• Sodium pyruvate
• Phenol Red

The complete formulation is available.

Gibco™ Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Product Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced RPMI 1640 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

iAdvanced Media: Reduced Sera Solutions. Quest, 2004. 1.3:26-30.

사양

세포주Sp2, Vero, Raji, Daudi, and Jurkat

분류Animal Origin

농도1 X

형태Liquid

제품 유형RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)

Serum LevelReduced Serum

유통 기한12 Months From Date of Manufacture

멸균Sterile-filtered

Sterilization MethodSterile-filtered

첨가제 포함Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)

첨가제 없음No Glutamine, No HEPES

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™

수량500 mL

배송 조건Room Temperature

Unit SizeEach