Thermo Fisher Scientific Granzyme B Mouse ProcartaPlex Simplex Kit

Invitrogen™ Granzyme B Mouse ProcartaPlex™ Simplex Kit

The Mouse Granzyme B Simplex ProcartaPlex Kit measures Granzyme B protein and is designed to be combinable with other Simplex kits, allowing creation of custom multiplex panels utilizing Luminex xMAP technology for protein detection and quantitation.

When combining multiple Simplex kits (i.e., when not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate multiple protein targets simultaneously using a Luminex instrument.

• Requires as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant
• Provides analyzed results within four hours

Features

• More results per sample — measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology — highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures identifiable by Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, MAGPIX).
Similar to a sandwich ELISA, matched antibody pairs identify the protein of interest. Each bead is labeled with antibodies specific for a single target protein, and bound proteins are detected with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE).

Unlike traditional ELISA, the capture antibody in ProcartaPlex assays is conjugated to a bead and not adsorbed to the microplate well, allowing reagents to remain free-floating in solution. Detection is performed by Luminex instruments using dual-laser systems to distinguish bead signatures and quantify RPE fluorescence proportional to protein concentration.

ProcartaPlex multiplex assays can profile more target proteins using significantly less sample and time compared to traditional ELISA methods.

ProcartaPlex multiplex panels are available for multiple species, including human, mouse, rat, nonhuman primate, porcine, and canine.
For more information, visit thermofisher.com/procartaplex.

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