Thermo Fisher Scientific alpha Tubulin Monoclonal Antibody (DM1A)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 145 publications 145 publications

Immunohistochemistry (IHC)

-

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 29 publications 29 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mg

View 1 publication 1 publication

Immunomicroscopy (IM)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Amphibian, Bovine, Dog, Chicken, Gerbil, Guinea pig, Human, Mouse, Pig, Rat

Published species

Amphibian, Bovine, Dog, Fruit fly, Hamster, Human, Mouse, Non-human primate, Pig, Protozoa, Rat, Sheep, Xenopus, Zebrafish

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

DM1A

Immunogen

Native chick brain microtubules if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Tubulin,uniProtId:P02552-1,ncbiNodeId:9031,antigenRange:1-412,antigenLength:412,antigenImageFileName:62204_alpha_Tubulin_P02552-1_House_mouse.svg,antigenImageFileNamePDP:62204_alpha_Tubulin_P02552-1_House_mouse_PDP.jpeg,sortOrder:11},{targetFamily:alpha Tubulin,uniProtId:P08070-1,ncbiNodeId:9031,antigenRange:1-446,antigenLength:446,antigenImageFileName:62204_alpha_Tubulin_P08070-1_House_mouse.svg,antigenImageFileNamePDP:62204_alpha_Tubulin_P08070-1_House_mouse_PDP.jpeg,sortOrder:12},{targetFamily:alpha Tubulin,uniProtId:P09643-1,ncbiNodeId:9031,antigenRange:1-322,antigenLength:322,antigenImageFileName:62204_alpha_Tubulin_P09643-1_House_mouse.svg,antigenImageFileNamePDP:62204_alpha_Tubulin_P09643-1_House_mouse_PDP.jpeg,sortOrder:13},{targetFamily:alpha Tubulin,uniProtId:P09644-1,ncbiNodeId:9031,antigenRange:1-448,antigenLength:448,antigenImageFileName:62204_alpha_Tubulin_P09644-1_House_mouse.svg,antigenImageFileNamePDP:62204_alpha_Tubulin_P09644-1_House_mouse_PDP.jpeg,sortOrder:14},{targetFamily:alpha Tubulin,uniProtId:P09645-1,ncbiNodeId:9031,antigenRange:1-324,antigenLength:324,antigenImageFileName:62204_alpha_Tubulin_P09645-1_House_mouse.svg,antigenImageFileNamePDP:62204_alpha_Tubulin_P09645-1_House_mouse_PDP.jpeg,sortOrder:15}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1965960

Product Specific Information

62204 detects alpha tubulin in human, mouse, rat, bovine, porcine, chicken, amphibian, gerbil and guinea pig samples.

62204 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, electron microscopy, immunocytochemistry and immunofluorescent applications.

The 62204 immunogen is native chick brain microtubules. This antibody recognizes an epitope within amino acids 426-450 of alpha tubulin.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.