Thermo Fisher Scientific IDH1 Polyclonal Antibody
Thermo Fisher Scientific의 IDH1 폴리클로날 항체는 Human, Mouse, Rat에 반응하며 Western blot, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제된 액상 항체로, 안정적인 보관 및 높은 특이성을 제공합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunoprecipitation (IP) | 1:100–1:1,000 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Cynomolgus monkey, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 30–337 of Human IDH1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.69 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545682 |
Product Specific Information
- Recommended positive controls: A431, HepG2, HeLa, Huh-7, Neuro2A, C8D30, NIH-3T3, Raw 264.7, C2Cl2, DDDDk-tagged IDH1-transfected 293T
- Predicted reactivity: Mouse (96%), Rat (97%), Zebrafish (85%), Xenopus laevis (88%), Sheep (95%), Rhesus Monkey (100%), Bovine (95%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses: one utilizes NAD(+) and the other NADP(+) as electron acceptors. Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent forms localized to the mitochondrial matrix, and two NADP(+)-dependent forms, one mitochondrial and the other cytosolic.
The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes, containing a PTS-1 peroxisomal targeting signal sequence. Its presence in peroxisomes suggests roles in NADPH regeneration for intraperoxisomal reductions and peroxisomal reactions consuming 2-oxoglutarate, such as alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme contributes significantly to cytoplasmic NADPH production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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