Thermo Fisher Scientific UCP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 33 publications 33 publications

Immunohistochemistry (IHC)

-

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 6 publications 6 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Cat, Ferret, Human, Mouse, Rat

Published species

Human, Mouse, Non-human primate, Rat, Rhesus monkey

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, conjugated to KLH, corresponding to residues 145-159 of human UCP1, with an N-terminal added cysteine. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:UCP1,uniProtId:P25874-1,ncbiNodeId:9606,antigenRange:145-159,antigenLength:307,antigenImageFileName:PA1-24894_UCP1_P25874-1_Rabbit.svg,antigenImageFileNamePDP:PA1-24894_UCP1_P25874-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.3 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2241459

Product Specific Information

PA1-24894 detects UCP1 from human, mouse, and rat samples, and does not cross-react with UCP2 or UCP3. PA1-24894 is expected to cross react with bovine (93% conserved), canine (93% conserved), Macaque monkey (100% conserved), rabbit (93% conserved), and ovine (93% conserved) due to sequence homology.

By Western Blot this antibody detects a band of approximately 32 kDa.

Positive Control: Brown adipose tissue.

Target Information

Mitochondrial uncoupling proteins are members of the family of mitochondrial anion carrier proteins. UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.