Thermo Fisher Scientific SPAG5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Protein corresonding to Human SPAG5. Recombinant protein control fragment (Product #RP-93178). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SPAG5,uniProtId:Q96R06-1,ncbiNodeId:9606,antigenRange:843-958,antigenLength:1193,antigenImageFileName:PA5-110877_SPAG5_Q96R06-1_Rabbit.svg,antigenImageFileNamePDP:PA5-110877_SPAG5_Q96R06-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2856288

Product Specific Information

Immunogen sequence: DTVENLTAKL ASTIADNQEQ DLEKTRQYSQ KLGLLTEQLQ SLTLFLQTKL KEKTEQETLL LSTACPPTQE HPLPNDRTFL GSILTAVADE EPESTPVPLL GSDKSAFTRV ASMVSL

Target Information

SPAG5 (sperm associated antigen 5), also known as MAP126, hMAP126, DEEPEST or Astrin, is a 1,193 amino acid protein that is expressed at high levels in testis and at lower levels in liver, placenta, pancreas, colon and thymus. Localized specifically to spindle poles during prophase and throughout the spindle during metaphase and anaphase, SPAG5 is essential for proper spindle formation and is thought to be involved in the dynamic and functional regulation of mitotic spindles. Additionally, SPAG5 plays a role in sister chromatid cohesion and subsequent chromatid separation and, via its association with chromatids, is an important regulator of chromosome integrity. SPAG5 has a long stalk and a globular head domain through which if forms a homodimer that is characterized by aster-like structures. Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.