Thermo Fisher Scientific SLAMF7/CS1/CD319 Monoclonal Antibody (SLAMF7, 3649)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed):
  Incubate antibody for 30 minutes at room temperature.
  Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

SLAMF7 contains one Ig-like C2-type (immunoglobulin-like) domain. Isoform 1 mediates NK cell activation through a SAP-independent ERK-mediated pathway and may play a role in lymphocyte adhesion. Isoform 3 does not mediate activation. SAP can bind the cytoplasmic tail of isoform 1 when phosphorylated in the presence of Fyn (in vitro).
SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung, and trachea. Expression occurs in NK cells, activated B-cells, and NK-cell lines but not in promyelocytic, B-, or T-cell lines. Isoform 3 is expressed at a much lower level than isoform 1. There are three named isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.