Thermo Fisher Scientific NSE Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:300

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:200–1:400

Product Specifications

Target Information

Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis, and the reverse reaction in gluconeogenesis. NSE is highly stable in biological fluids and diffuses easily into the extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.

NSE is one of three mammalian enolases (ENO1, ENO2, ENO3), also known as enolase alpha, beta, and gamma.

• Alpha-subunit: expressed in most tissues
• Beta-subunit: expressed in muscle
• Gamma-subunit: expressed primarily in neurons and neuroendocrine cells

Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Antibodies to NSE are useful for identifying neuronal cell bodies, developing neuronal lineage, and neuroendocrine cells. Release of NSE into CSF and blood serves as a biomarker of neuronal injury.

Clinically, NSE is used as a sensitive marker for neuronal damage in neurological disorders such as stroke, hypoxic brain injury, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Elevated NSE levels are also found in certain neoplasias, including pediatric neuroblastoma and small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.