Thermo Fisher Scientific PHF7 Monoclonal Antibody (OTI1A2)
PHF7 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합합니다. Human 시료 반응성이 있으며, 액상 형태로 1 mg/mL 농도, -20°C 보관 조건을 가집니다. Spermatogenesis 연구용으로 최적화된 항체입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 155–381 of PHF7 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2725012 |
Target Information
Spermatogenesis is a complex process regulated by extracellular and intracellular factors, as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. In the testis, this gene is expressed in Sertoli cells but not germ cells. However, it is not expressed in patients exhibiting spermatogenic arrest at the spermatocyte stage. Spermatogenic arrest interrupts germ cell differentiation and may result in oligospermia or azoospermia.
The proteins encoded by this gene contain plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, which are believed to be involved in transcriptional regulation. This protein localizes to the nucleus of transfected cells and is implicated in transcriptional regulation of spermatogenesis. Two protein isoforms are encoded by transcript variants of this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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