Thermo Fisher Scientific HEF1 Monoclonal Antibody (2G9)
HEF1 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 적합합니다. Human, Mouse, Rat 시료에서 반응하며 Protein G로 정제된 액상 제품입니다. 세포 부착 및 신호전달 연구에 유용합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific HEF1 Monoclonal Antibody (2G9)
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:6,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | – |
| Immunocytochemistry (ICC/IF) | Assay-dependent | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published species | Human, Non-human primate |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2G9 |
| Immunogen | Fusion protein corresponding to amino acids 82–398 of Human HEF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2149486 |
Product Specific Information
This antibody detects HEF1 in human, mouse, and rat samples. It does not recognize p130cas and has not been tested on Sin1. The antibody primarily detects HEF1 localized at focal adhesion sites.
Target Information
HEF-1 is a multifunctional protein involved in integrin-based signaling that influences cell motility, growth, apoptosis, and oncogenic transformation. The Cas family of docking proteins are substrates of focal adhesion kinase (FAK) and Src family tyrosine kinases, both key targets in drug development. HEF1 overproduction increases expression of proteins linked to motility and invasiveness, such as metalloproteinases, MLCK, p160ROCK, and ErbB1, and mediates apoptosis in epithelial-derived cell lines like MCF7 and HeLa. Overexpression of BCAR1 (p130Cas), a related protein, has been associated with tamoxifen resistance, underscoring the importance of this protein family in cancer prognosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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