Thermo Fisher Scientific DYKDDDDK Polyclonal Antibody
DYKDDDDK(FLAG) 태그 인식용 Thermo Fisher Scientific의 rabbit polyclonal antibody로, WB, IHC, ELISA, IP 등 다양한 분석에 사용 가능. 높은 특이성과 재현성을 제공하며, 단백질 발현 및 정량 연구에 적합. 연구용으로만 사용.
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Thermo Fisher Scientific DYKDDDDK Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:20,000–1:40,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the carboxy terminal end to facilitate coupling. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against the FLAG™ motif and is useful in determining its presence in various assays.
This polyclonal anti-FLAG™ tag antibody detects over-expressed proteins containing the FLAG™ epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence commonly used as a protein tag to facilitate protein purification, detection, and localization.
The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing easy identification and isolation using anti-FLAG antibodies.
The DYKDDDDK tag is widely used in molecular biology and biochemistry research for protein-protein interaction studies, protein expression analysis, and protein localization studies.
The FLAG tag structure is optimized for compatibility with attached proteins, being more hydrophilic than other epitope tags and therefore less likely to denature or inactivate proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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