Thermo Fisher Scientific eIF3h Polyclonal Antibody
Rabbit polyclonal antibody targeting eIF3h for WB, IHC, and ICC applications. Reacts with human and mouse. Affinity-purified, liquid form, unconjugated. Supplied at 0.5 mg/mL in PBS with sucrose and sodium azide. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.2–2 µg/mL |
| Immunohistochemistry (IHC) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:500 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide directed towards the N-terminal of human EIF3H (aa 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS with 2% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2610349 |
Product Specific Information
Peptide sequence:
MASRKEGTGS TATSSSSTAG AAGKGKGKGG SGDSAVKQVQ IDGLVVLKII
Sequence homology:
Cow: 93%
Dog: 86%
Guinea Pig: 86%
Horse: 86%
Human: 100%
Mouse: 100%
Pig: 93%
Rabbit: 86%
Rat: 100%
Target Information
eIF3H is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S pre-initiation complex (43S PIC). It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. It specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, differentiation, and apoptosis, using different RNA stem-loop binding modes to exert translational activation or repression.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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