Thermo Fisher Scientific GPR149 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) [IHC (P)]

• Tested Dilution: 20 µg/mL
• 참고: Heat-induced antigen retrieval in pH 6.0 citrate buffer 필요. 1차 항체 후 biotinylated 2차 항체, alkaline phosphatase-streptavidin 및 chromogen 처리.

ELISA

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Percent identity with other species by BLAST analysis:

• Human, Gorilla, Gibbon: 100%
• Elephant: 94%
• Monkey, Marmoset: 88%
• Bat: 81%

Target Information

This gene encodes a seven-transmembrane G protein coupled receptor (GPCR) class A family member. Although categorized as a class A GPCR, the encoded protein lacks the first two charged amino acids of the highly conserved Asp-Arg-Tyr (DRY) motif found in the third transmembrane helix of class A receptors, which is important for efficient G protein-coupled signal transduction.

Mice with a knockout of the orthologous gene are viable and have normal maturation of the ovarian follicle, but show enhanced fertility and ovulation.

All GPCRs have a common structural architecture consisting of seven transmembrane alpha-helices interconnected by three extracellular and three intracellular loops. A general feature of GPCR signaling is agonist-induced conformational changes in the receptor, leading to activation of heterotrimeric G proteins (G-alpha, G-beta, G-gamma subunits). The activated G proteins bind and activate downstream effector proteins, generating second messengers that mediate a broad range of cellular and physiological processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.