Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), Brilliant Ultra Violet 615, eBioscience

Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), Brilliant Ultra Violet 615, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.25 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells, activated B cells, and activated T cells.
CD80 binds with high affinity to CD28 and CD152 (CTLA-4) on T cells, playing a crucial role in T-B cell communication and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes; ≤ 0.25 µg per test (100 µL final volume)
  • Cell number: 10⁵–10⁸ cells/test
  • Antibody titration recommended for optimal performance

Brilliant Ultra Violet™ 615 (BUV615) is a tandem dye emitting at 615 nm, suitable for cytometers equipped with UV (355 nm) lasers.
Ensure instrument capability before use.

When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.

• Light Sensitivity: Protect vial and samples from light (photo-induced oxidation risk)
• Fixation: Samples may be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal effect on brightness or FRET efficiency.
• Excitation: 350 nm
• Emission: 615 nm
• Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands for the T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152).
Both are expressed on professional antigen-presenting cells and essential for T cell activation.
CD80 is rapidly induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, follicular B lymphoma cells, T cells, and monocytes.
It provides regulatory signals for T lymphocytes via binding to CD28 and CTLA4.
Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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