Thermo Fisher Scientific eIF3c Polyclonal Antibody
Human eIF3c 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 IHC에 적합. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. PBS 기반 저장용액에 40% 글리세롤 포함, 연구용으로만 사용.
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Thermo Fisher Scientific eIF3c Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human eIF3c. Recombinant protein control fragment (Product #RP-104461). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2640961 |
Product Specific Information
Immunogen sequence:
NELMDILFAN PNIFVGENIL EESENLHNAD QPLRVRGCIL TLVERMDEEF TKIMQNTDPH SQEYVEHLKD EAQVCAIIER VQRYLEEKGT TE
Highest antigen sequence identity to orthologs:
- Mouse: 95%
- Rat: 95%
Target Information
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S preinitiation complex (43S PIC).
It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition.
The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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