Thermo Fisher Scientific PHD4 Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:2,500

Product Specifications

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen.
HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen.
In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction.
Low oxygen levels cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.
Prolyl hydroxylase 4 specifically catalyzes the posttranslational formation of 4-hydroxyproline in HIF-alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, may target HIF for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.