Takara SacI restriction enzyme

SacI restriction site:

G AGCT | C
C | TCGA G

Supplied buffer: L

Effect of DNA methylation: enzyme activity is not affected by CG methylase

Reaction temperature: 37°C

More Information

• Source: Streptomyces achromogenes
• Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
• Substrate for unit definition: lambda DNA
• Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or high concentrations of glycerol
• Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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