Thermo Scientific™

SuperSignal™ West HisProbe Kit

The Thermo Scientific SuperSignal West HisProbe Kit contains HisProbe-HRP Conjugate, as well as reagents required for detection of polyhistidine-tagged protein자세히 알아보기

The Thermo Scientific SuperSignal West HisProbe Kit contains HisProbe-HRP Conjugate, as well as reagents required for detection of polyhistidine-tagged protein by western blotting. HisProbe-HRP Conjugate is a nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.

Features of the SuperSignal West HisProbe Kit:

• Fast—His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
• Economical—no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies
• High specificity—HisProbe-HRP can recognize his-tags independent of adjacent tags.
• Less steric hindrance—HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies

Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC). The Thermo Scientific SuperSignal West HisProbe Kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate.

Related Products
HisProbe™-HRP Conjugate

사양

설명Pierce SuperSignal West HisProbe Kit

제품 유형HisProbe Detection Kit

검출 방법Chemiluminescence

용도(애플리케이션)Western Blot

Membrane CompatibilityNitrocellulose, PVDF

제품라인HisProbe™, SuperSignal™

수량685 mL

콘주게이트HRP

SubstrateHorseradish Peroxidase

반응성All species

충분62 mini blots or 5000 sq cm membrane

Unit SizeEach