Thermo Fisher Scientific CD7 Monoclonal Antibody (4H9), FITC, eBioscience

Thermo Fisher Scientific CD7 Monoclonal Antibody (4H9), FITC, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.125 µg)/test

Publications

• View 5 publications

Product Specifications

Available Formats

• APC
• PE
• PerCP-eFluor 710
• Custom conjugation available on request

Product Specific Information

This 4H9 monoclonal antibody reacts with human CD7 (Leu-9, gp40), a 40-kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is expressed on peripheral blood T lymphocytes and natural killer cells, as well as on lymphoid and myeloid lineage precursors during adult hematopoiesis. It is involved in T cell activation through association with CD3 and CD45.

CD7 is also detected in T cell-derived leukemias and lymphomas. Because CD7 expression is greater on CD4+ T cells in classical Hodgkin’s lymphoma than in benign reactive lymphadenopathy, it may serve as a diagnostic marker for Hodgkin’s lymphoma.

Blocking assays demonstrate that this clone recognizes the same epitope as clone 124-1D1.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.125 µg) per test. A test is defined as the amount of antibody staining a sample in a final volume of 100 µL.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily. The N-terminal region (amino acids 1–107) shows high homology to Ig kappa-L chains, while the C-terminal region is proline-rich and forms a stalk projecting the Ig domain. CD7 is expressed on most immature and mature T lymphocytes, T cell leukemia cells, NK cells, and a small subset of B cells. It positively modulates T cell and NK cell activity through calcium fluxes, adhesion molecule expression, cytokine secretion, and proliferation. CD7 associates directly with phosphoinositol 3′-kinase, and its ligation induces D-3 phosphoinositide production and tyrosine phosphorylation. CD7 expression serves as an important marker in leukemia diagnostics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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