Thermo Fisher Scientific SNAP alpha Polyclonal Antibody
SNAP alpha 단백질을 인식하는 Rabbit Polyclonal Antibody로 인간, 마우스, 랫트에 반응. WB, IHC, ELISA 등 다양한 응용 가능. 고순도 Affinity chromatography 정제, 50% glycerol 저장 buffer 포함. 연구용으로만 사용.
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Thermo Fisher Scientific SNAP alpha Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–295 of human Alpha SNAP (NP_0038182) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901742 |
Product Specific Information
Positive Samples: HL-60, THP-1, HeLa, Jurkat, Mouse brain, Mouse lung, Mouse heart, Mouse pancreas, Rat liver
Immunogen Sequence:
MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
Target Information
SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion, and transcytotic vesicles-to-plasma membrane transport.
Vesicle-to-target membrane docking occurs when the vesicle SNARE binds to its cognate target membrane SNARE. α-SNAP (or β-SNAP in brain) then binds to this docking complex and mediates the binding of NSF, forming a 20S fusion particle. Once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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