Thermo Fisher Scientific Human EGF, Animal-Free Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

-

Immunomicroscopy (IM)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1,143 publications 1,143 publications

Miscellaneous PubMed (Misc)

-

View 187 publications 187 publications

Immunostaining (IS)

-

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Amphibian, Bovine, Cat, Chicken, Chordate, Dog, Hamster, Horse, Human, Mouse, Non-human primate, Panda, Pig, Rabbit, Rat, Reptile, Sheep, Zebrafish

Expression System

E. coli

Amino acid sequence

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Molecular weight

6.2 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human EGF is a 6.2 kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide bonds.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.