
Thermo Fisher Scientific Phospho-A Raf (Ser299) Polyclonal Antibody
A-Raf 단백질의 Ser299 인산화 부위를 인식하는 rabbit polyclonal antibody. WB, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. Protein A로 정제된 1 mg/mL 액상 형태. -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human A-Raf around the phosphorylation site of Ser299 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Several serine/threonine protein kinases act as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity and is broadly expressed in nearly all tested cell lines. It is the cellular homolog of v-Raf, the transforming gene product of the murine sarcoma virus strain 3611. The unregulated kinase activity of v-Raf is associated with transformation and mitogenesis, whereas Raf-1 activity is normally suppressed by its regulatory N-terminal domain.
A-Raf, another member of the Raf gene family, shows substantial homology with Raf-1 in the kinase domain but less homology elsewhere. A-Raf expression is highest in urogenital tissues and kidney and lowest in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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