Thermo Fisher Scientific Cytokeratin 20 (KRT20) Recombinant Rabbit Monoclonal Antibody (KRT20, 3129R)
Cytokeratin 20 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 IHC(P) 실험에 적합합니다. 인간 시료에 반응하며, Protein A/G로 정제된 액상 항체입니다. PBS 버퍼에 0.05% BSA 및 sodium azide를 포함하고 4°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | KRT20, 3129R |
| Immunogen | Recombinant fragment of human KRT20 protein (around aa 196–323) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues, heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes before staining.
Target Information
The protein encoded by this gene belongs to the keratin family, which provides structural integrity to epithelial cells. Keratins are divided into cytokeratins and hair keratins. Type I cytokeratins consist of acidic proteins that form heterotypic pairs. Cytokeratin 20 is a major cellular protein in mature enterocytes and goblet cells, specifically expressed in gastric and intestinal mucosa. The type I cytokeratin genes are located on chromosome 17q12–q21.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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